Recipe of the Week – Week 7: Janus Green B

Continuing our new series of chemical recipes, we bring you week 7 – Janus Green B (C₃₀H₃₁CIN₆).

Remember that when preparing any of our recipes, you should always wear appropriate PPE.

What is Janus Green B?

Janus Green B is a dye and vital stain (a stain that can be applied to living cells without killing them) that changes colour to indicate the amount of oxygen present in an aqueous solution. A blue colour indicates the presence of oxygen whereas pink indicates absence. Before application, the powder is a dark green/black colour.

It is also known as Union Green B and Diazin Green S, and has been used in surgical and diagnostic procedures involving mitochondria, fungi, nucleic acids and chromosomes, among others.

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Scan the QR codes for classroom experiments to follow with your safe and prepared Janus Green B.